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Introduction

The following manual describes standard operating procedures for the operation of the Star Island Wastewater Treatment Facility (SICWTF) and implementation of process control analyses at the SICWTF’s laboratory.

Open-up Manual 4

A. Who to Call 4

B. Where to Find Everything. 5

C. Open-up Checklist 6

1. Main Pump Station. 7

2. Sludge Tanks. 7

3. Drying Beds. 7

4. Biosolids. 8

5. Doctor’s Pump Station. 9

6. Sewage/Effluent Lines. 9

7. EQ Basin. 9

8. SBR Basin. 10

9. Chlorine Contact Tanks. 10

10. Effluent Pump Station. 11

11. Laboratory. 11

12. W(astewater) U(tility) B(uilding) 12

13. Garage. 12

14. Off-Season Septic. 12

D. Open-Up Procedures. 14

E. Scheduling. 17

Close-up Manual 18

A. Gearing up for Close-up. 18

Bulk Chemicals. 18

Sludge Tanks and Drying Beds. 19

Chlorine Contact Tanks (CCT) 19

Equalization Basin (EQ) 19

B. Where to Store Everything. 20

C. Close-up Checklist 21

1. Doctor’s Pump Station. 22

2. Equalization Basin. 22

3. Laboratory. 22

4. Sequencing Batch Reactor 23

5. Chlorine Contact Tanks. 24

6. Effluent Pump Station. 24

7. Sludge Tanks. 24

8. Sludge Lines. 24

9. Drying Beds. 25

10. Effluent Line. 25

11. Drying Bed Supernatant Well 25

12. Biosolids. 25

Morning Rounds. 26

Process Testing. 29

Process Test Sampling. 29

Dissolved Oxygen (DO) Profile. 31

Total Iron mg/L. 34

Mixed Liquor Suspended Solids. 38

Waste Activated Sludge. 43

Nitrate. 48

Turbidity. 52

Salinity. 55

Oxygen Uptake Rate (OUR) 56

Thirty Minute Settleability. 60

Mixed Liquor pH.. 63

Process Data. 66

= Open-up Manual=

A. Who to CallEdit

Contact

#

email

good for . . .

poo phone

603-817-4962

wtf@starisland.org

Mike Becker, Eastern Analytical

800-287-0525

scheduling biosolids and setting up testing schedule (enterococci, sometimes BOD) for season

Ralph Cross, Economy Lab Services

508-951-1272

equipment calibration, fax service requests to 508-636-7405

Bob Urban

603-863-6478

urbanrc@nhvt.net

wastewater consultant

Aqua-Aerobic Systems

815-654-2501 (phone)

solutions@aqua-aerobic.com

aquacam service, repair, questions, etc.

Larry Fry

815/639-4405 (Toll Free 800/940-5008, ext. 4405 )

LFRY@aqua-aerobic.com

technical service

Tim Lamont

TLAMONT@aqua-aerobic.com

purchasing/customer service

Stephanie Larson

1-603-271-1493

Stephanie.Larson@des.nh.gov

reporting exceedances, questions regarding reporting, DMR's, MOR's, etc.

Joy Hilton

1-617-918-1877

Hilton.Joy@epamail.epa.gov

reporting exceedances, DMR forms

==B. Where to Find Everything ==

Edit

EMB Loft

Reagents

Moisture/freezing sensitive supplies

Sensitive equipment

EMB Back Rooms

Autoclave

Back-up autoclave

Leftover distilled water

WUB

Sludge buckets

Sampling equipment

Bucket

Sludge judge

Sludge nabber

Garage Loft Above Big Door

Broken glass containers

Carboys

Extra BOD bottles

Ancient colorimeters

Garage Loft to Right of Normal Door

Fencing

Garage

Pumps

Main pump station set-up

Effluent pump station set-up

Main pump station alarm tester

Sigma sampler

Alley

Green hoses

Tools

Lab

Glassware

Vacuum pump

Microscope

Dessicator

Refrigerators

Incubators

Bod

Tc

Oven

Air conditioner

Star Island Office

Permit binders

Process binders

Correspondence

WTF office: Cottage D

Permit information.

Correspondence

Hoop Fort

Back-up Mixer Motors and Aqua-Aerobics equipment

Pumps

Edit

==C. Open-up Checklist==

See Lists Specific to Areas

All equipment manuals are located on island in the file cabinet in the garage.

Area

1. Main Pump Station

2. Sludge Tanks

3. Drying Beds

4. Biosolids

5. Doctor’s Pump Station

6. Sewage/Effluent Lines

7. EQ Basin

8. SBR Basin

9. Chlorine Contact Tanks

10. Effluent Pump Station

11. Laboratory

12. W(astewater) U(tility) B(uilding)

13. Garage

14. Off-Season Septic

15. Pumps

Maintenance Complete

_____

_____

_____

_____

_____

_____

_____

_____

_____

_____

_____

_____

_____

_____

_____

===1. Main Pump Station===

Part

Maintenance

completed

initials

Main Pump Station

follow pump-specific maintenance

Make sure impeller rotates when pump is plugged in

Make sure impeller rotates in direction specified on pump

re-install pumps

inspect float switches for proper operation

re-install overflow pumps

zero pump timers

2. Sludge TanksEdit

Part

Maintenance

Completed

Initials

sludge tanks

attach appropriate green hoses to sludge pumps (dominators)

Locate sludge pumps.

follow pump-specific maintenance

Make sure impellers rotate when pumps are plugged in

Make sure impellers rotate in direction specified on pumps

drop sludge pumps into sludge tanks

3. Drying BedsEdit

Part

Maintenance

completed

initials

Drying Beds

Unscrew tops from bases

Inspect and make repairs as necessary to the following: plastic tops, boards in bases, ropes, pulleys, etc.

Inspect sand levels and add if necessary.

sludge lines

inspect and repair as necessary

supernatant lines

inspect and repair as necessary

well

inspect and repair as necessary

4. BiosolidsEdit

1. Waste Management provides a sludge dumpster after sludge is tested and approved for disposal.

a) Requirements are listed in the EPA’s Sludge Compliance Guidance.

i) http://www.epa.gov/ne/npdes/permits/generic/sludgeguidance.pdf

Part 2.3.4. Scenario No. 4 (pg.55/70)

2. Sludge is tested for the following:

a) TCLP Metals – (RCRA-8)

b) Paint Filter

c) TCLP Volatiles

d) TCLP Semi-Volatiles

e) Percent Solids (Total)

3. Sludge is sent to Eastern Analytical for analysis.

a) Two eight ounce samples and one four ounce sample.

i) All samples must be kept in glass bottles.

ii) Four ounce sample is light sensitive and must be collected in an iii) amber colored bottle or must be wrapped in foil.

b) Place bottle orders with Eastern Analytical.

i) Chain-of-Custody Record or Autochain will be provided.

c) Contact Mike Becker at Eastern Analytical (see Contact List).

Edit

Edit

Edit

Edit

Edit

Edit

Edit

Edit

===5. Doctor’s Pump Station===

Part

Maintenance

completed

initials

Doctor's Pump Station

follow pump-specific maintenance

Make sure impeller rotates when pump is plugged in

Make sure impeller rotates in direction specified on pump

re-install pumps

inspect float switches for proper operation

re-install overflow pumps

zero pump timers

Edit

6. Sewage/Effluent LinesEdit

Part

Maintenance

completed

initials

sewage/effluent lines

trace for breaks and leaks

repair breaks and leaks

ensure all connections are in place

Edit

7. EQ BasinEdit

Part

Maintenance

Completed

Initials

EQ Tank

Make sure cables are connected to unit and walls

Inspect ladders: repair/replace if necessary.

Aquacam power section

Spin impeller.

SBR feed pump

Follow pump specific maintenance.

Make sure impeller rotates when pump is plugged in

Make sure impeller rotates in direction specified on pump

put pump into place in EQ: thread pvc pipe to pump output and attach to remainder of set-up w/rubber coupling.

Air Hose

Inspect connections and hose for possible wear.

Plug

make sure plug is secure in sump hole.

Edit

===8. SBR Basin===

Part

Maintenance

Completed

Initials

SBR Tank

Make sure cables are connected to unit and walls

Inspect ladders: repair/replace if necessary.

AquacamD power section

Spin impeller.

Decanter Linear Actuator

Inspect. Requires no maintenance. Should operate quietly and smoothly. If not, replace.

Check travel and proper weir opening.

Limit Switches

Make sure properly set for actuator travel start and stop positions --- don't want the weir to close to tightly or too loosely

Decanter Drum

Inspect seal on decanter drum: replace if necessary.

Sludge Pump

Follow pump specific maintenance.

Make sure impeller rotates when pump is plugged in

Make sure impeller rotates in direction specified on pump

install pump in SBR

Decanter Discharge Hose and Flexible Air Lines

Inspect connections and hose for possible wear.

Make sure hoses have clearance and won't bind during operation.

Valve actuators

inspect for proper operation: replace if necessary.

Plug

Make sure plug is in place in sump hole.

Edit

9. Chlorine Contact TanksEdit

Part

Maintenance

Completed

Initials

sumps

put plugs in place

outside drainages

put caps in place

disinfection

put both bleach lines in place

dechlorination

put bisulfite line into discharge of one chlorine contact tank

Edit

10. Effluent Pump StationEdit

Part

Maintenance

completed

initials

Effluent Pump Station

follow pump specific maintenance

Make sure impeller rotates when pump is plugged in

Make sure impeller rotates in direction specified on pump

install pump

inspect float switches for proper operation

zero pump timer

Edit

11. LaboratoryEdit

Task Finished

  1. Clean thouroughly ___
    1. Lights ___
    2. shelves and counters ___
    3. sink ___
    4. floor ___
  2. Set up drying rack with lab mat. ___
  3. Unpack glassware. ___
    1. Wash ___
    2. Set up ___
  4. Take items out of storage ___
    1. See “storage” section
  5. Set up and turn on equipment. ___
    1. Purchase batteries for necessary equipment. ___
  6. Schedule equipment calibration (for late May) ___
  7. Inventory remaining reagents and supplies ___
    1. purchase what is needed ___
    2. See inventory binder and spreadsheets
  8. Set up broken glass container ___

12. W(astewater) U(tility) B(uilding)Edit

Part

Maintenance

Completed

Initials

Chemicals

organize remainders

dispose of remainders as necessary

order

Chemical Pumps

test for proper operation

set-up (2 bleach, 1 bisulfite)

inventory spare parts and order what's necessary.

Bleach

connect intake lines to bleach pumps (2)

Connect discharge lines

Sodium bisulfite

Connect intake lines to bisulfite pump (1)

Connect discharge line

13. GarageEdit

Part

Maintenance

Completed

Initials

tools

check against inventory

grease

organize

14. Off-Season SepticEdit

Part

Maintenance

Completed

Initials

After all inflow has been directed to the EQ:

Poop Line

remove and store (make sure to change flow valves).

doctor's holding tank

transfer all sewage to doctor's pump station.

septic tank

transfer all sewage to doctor's pump station or EQ.

15. Pumps

Part

Maintenance

completed

initials

all pumps

follow pump-specific maintenance

Make sure impeller rotates when pump is plugged in

Make sure impeller rotates in direction specified on pump

Edit

Edit

D. Open-Up ProceduresEdit

June 2008

Star Island Wastewater Treatment Facility

1. When drying bed maintenance is complete

a) Remove remaining sludge from beds

2. When sludge tank maintenance is complete

b) Flow remaining sludge to drying beds

3. When EQ maintenance is complete

a) Take septic system off line.

i) Make sure main pump station flows to EQ.

Turn valve at Sprague septic tank.

ii) Make sure flow is directed into doctor’s pump station.

Turn valve behind doctor’s.

iii) Direct flow from the barns into the EQ.

Turn valve at southwest corner of SBR.

4. When EQ is filled above low water line (3’)

a) Set EQ basin mixer to run constantly

b) Monitor dissolved oxygen level

i) Keep above 2.0mg/L.

5. If foam from soap and bacterial processes becomes an issue

a) Adjust on/off cycle times (45 off/15 on works pretty well)

i) Reduce foam

ii) Maintain DO of 2.0mg/L

b) Spray foam down with hose.

6. Add 10 lb. of D 500C bioaugmentation to EQ.

a) Add 1lb bioaug/day to the EQ until wastewater is transferred to the SBR.

7. Allow EQ basin to fill with wastewater to the overflow port to the SBR.

a) At this level the basin should contain 34,467 gallons (16’ x 24’ x 12’ x 7.48).

In 2008 it took 14 days to fill the EQ to 10’.

8. Empty the EQ into the SBR using the SBR feed pump (on hand).

a) Turn SBR mixer motor to auto when AquacamD is floating.

9. Set SBR cycle times to the following:

MF: 35

RF: 70

R: 63

S: 70

D: 50

10. Turn aerator valve to auto.

11. Set aeration counters to the following:

Cycle #1: 38/5

Cycle #2-5: 30/5

12. Counters are initially set low

a) To maintain reasonable D.O.

b) Control foam that occurs during start up

c) Since limited organic loadings during start up

13. Begin taking D.O. profiles.

a) Maintain oxygen levels between 2 and 3mg/L.

b) Adjust as necessary.

14. Discontinue addition of bioaugmentation to the EQ.

15. Begin adding 1lb bioaugmentation/day to the SBR.

16. Turn SBR feed pump to auto.

17. Set the SBR feed pump to slowly fill the SBR.

a) Gives the process time to mature.

b) First decant should be at the beginning of a week so all required testing can be completed.

c) SBR feed pump has been set at 19/1 in the past.

SBR should fill in 8-12 days, depending on wastewater flow and EQ level.

18. Allow decant cycles to begin when SBR has reached 9.25’ (3” higher than low water level – 9.0’)

a) Decant valve to auto

b) Decant weir to auto----

c) Chlorination pumps to auto

d) Dechlorination pumps to auto

e) Effluent pump station to auto

f) Sludge Pump off

19. Make sure correct valves are open on chlorine contact tanks (CCT)

a) Discharge into only one

b) Make sure disinfection and de-chlorination systems are in place.

c) Be ready to take compliance samples for analysis.

20. Monitor Mixed Liquor Suspended Solids (MLSS) on a daily basis.

When at 1000mg/L begin wasting every other day.

Set sludge wasting at 1.0min/cycle

Target: 1500-2000mg/L.

When reached waste every day.

21. After decants begin and process matures:

Adjust aeration cycles.

Adjust cycle times as necessary.

Adjust SBR feed pump to maintain level in EQ.

Use tank dimensions to determine wastewater volumes.

Use pump rates and tank volumes to determine SBR feed pump times.

SBR feed pump rates

8’-10’: 80gpm

6’-8’: 65gpm

4’-6’: 50gpm

Target EQ level: approximately 4’ in the early morning.

This level will rise during the day.

Use tank dimensions to determine wastewater volumes

Use pump rates and tank volumes to determine SBR feed pump times.

E. SchedulingEdit

General Permit Testing Schedule

Sunday

Monday

Tuesday

Wednesday

Thursday

Friday

Saturday

testing

TRCPD, TRC, pH

TRCPD, TC, pH, TC

TRCPD, TC, pH, TC

TRCPD, TC, pH, TC

TRCPD, TC, pH, TC

TRCPD, TC, pH, TC, TSS

TRCPD, TRC, pH

sampling

T.C. 24 hr composite: first effluent

T.C. 24 hr composite: 2nd effluent, influent

T.C. enterococci

T.C.

T.C.

to Eastern Analytical

enterococci 24 hr composites for BOD analysis

Eastern Analytical provides the following analysis for the SICWTF:

Enterococci: weekly

BOD: weekly

Laboratory Water: with each batch of DI water

HPC (Heterotrophic Plate Count)

Conductivity

Biosolids: once/season

Decant schedule established around boat schedule.

Enterococci should be sampled as close to boat departure times as possible due to a very short hold time.

BOD composites sent to Eastern Analytical at the same time.

Sending enterococci and BOD to Eastern Analytical on Wednesday allows for further testing/sampling if necessary (permit week is Sunday to Saturday).

=Close-up Manual=

A. Gearing up for Close-upEdit

Bulk ChemicalsEdit

Bleach

1. Only one or two barrels of bleach should be left over the winter.

a) Bleach quickly loses potency (very short half life).

b) Bleach stored over the winter will not deliver adequate disinfection.

2. Make sure there is enough bleach to get through the rest of the season.

a) WTF and RO

If extra:

1. See if Appledore needs any.

2. Offer bleach to housekeeping.

3. Begin disposing of bleach.

a) See below.

How to dispose of bleach:

1. Pour small amounts of bleach into chlorine contact tank (CCT) in use during each decant cycle.

2. Keep chlorine contact tank (cct) that is not in use filled with effluent.

a) Pour bleach into this cct.

i) If bleach is potent, pour small amounts in.

See section on Chlorine Contact Tanks: this chlorinated effluent will be de-chlorinated with the decant when pumped into CCT in use. Be cautious with this to avoid chlorine exceedances.

3. Return empty Bleach barrels for refund.

a) 55-gal drum = $50

b) Coordinate drop-off/pick-up with Monson

Sodium Bisulfite

1. Sodium Bisulfite does not half-life quickly.

a) Will crystallize if stored for the winter.

b) Can still be used

i) More likely to clog pumps and lines.

ii) Less usable bisulfite

If extra:

1. Consolidate

a) May be able to reconstitute crystals during the following season.

2. Can also return sodium bisulfite drums for a refund.

Sludge Tanks and Drying Beds Edit

1. Make sure there is plenty of room for sludge when the SBR is empty

2. Stay on top of removing sludge

a) All summer, but especially during the beginning of September.

i) Don’t slack!

ii) At least two empty sludge tanks are needed to completely empty the SBR!

Chlorine Contact Tanks (CCT)Edit

1. Keep one CCT in use.

2. If full, empty other CCT.

a) Pump into operable CCT during decant.

3. Clean empty CCT

a) Spray with salt-water hose to remove dirt

b) Scrape baffles if necessary

c) Scrub out bottom

i) Leave salt-water hose running near the CCT inlet.

ii) Pushes dirt towards sump.

d) Pump salt-water into EQ.

Equalization Basin (EQ)Edit

1. Keep level in EQ low

a) Able to quickly empty into SBR

B. Where to Store EverythingEdit

EMB Loft

Reagents

Moisture/freezing sensitive supplies

Sensitive equipment

EMB Back Rooms

Autoclave

Back-up autoclave

Leftover distilled water

WUB

Sludge buckets

Sampling equipment

Bucket

Sludge judge

Sludge nabber

Garage Loft Above Big Door

Broken glass containers

Carboys

Extra BOD bottles

Ancient colorimeters

Garage Loft to Right of Normal Door

Fencing

Garage

Pumps

Main pump station set-up

Effluent pump station set-up

Main pump station alarm tester

Sigma sampler

Alley

Green hoses

Tools

Lab

Glassware

Vacuum pump

Microscope

Dessicator

Refrigerators

Incubators

Bod

Tc

Oven

Air conditioner

Star Island Office

Permit binders

Process binders

Correspondence

WTF office: Cottage D

Permit information.

Correspondence

Hoop Fort

Back-up Mixer Motors and Aqua-Aerobics equipment

Pumps

==C. Close-up Checklist==

See Area-Specific Maintenance Instructions in Close-up Manual.

All equipment manuals are located on island in the file cabinet in the garage.

Area

1. Doctor’s Pump Station

2. Equalization Basin

3. Laboratory

4. Sequencing Batch Reactor

5. Chlorine Contact Tanks

6. Effluent Pump Station

7. Sludge tanks

8. Sludge Lines

9. Drying beds

10. Effluent line

12. Drying bed supernatant well

13. Biosolids

Maintenance Complete

_____

_____

_____

_____

_____

_____

_____

_____

_____

_____

_____

_____

1. Doctor’s Pump StationEdit

Maintenance

Completed

Initials

remove pumps

cover

2. Equalization BasinEdit

Part

Maintenance

Completed

Initials

EQ tank

empty completely

loosen cables as EQ is emptied

clean tank: pressure wash if possible.

Aquacam Power Section

grease top unit: 9 pumps

grease lower unit: until grease discharges from outlet opposite grease pip.

Cover securely with tarp.

SBR feed pump

unthread pvc pipe from pump; leave in tank.

remove pump.

Plug

remove

Edit

3. LaboratoryEdit

1. Dispose of reagents and standards that will be expired by the following spring.

2. Inventory remaining reagents and standards.

Pack for warm storage

3. Store scale (and anything with digital display) with reagents.

4. Prepare all probes for winter storage.

5. Remove batteries from equipment.

a) Dispose.

6. Pack colorimeter, field DO meter, bench top DO meter, pH meter, and conductivity meter for warm storage.

7. Thoroughly clean lab.

8. Wash all glassware.

9. Store plastic bottles and beakers in bags on shelves.

10. Pack glass in Tupperware bins (cushion somehow).

11. Store dessicator and microscope in cabinet under benchtop.

12. Unplug all equipment.

13. Clean refrigerators, incubators, and ovens.

14. Dispose of all trash and recycling.

15. Clean autoclave and move to warm storage.

16. Dispose of de-ionized water: dump or move to Newtong for drinking/cooking.

4. Sequencing Batch ReactorEdit

Part

Maintenance

Completed

Initials

SBR Tank

empty completely

loosen cables as SBR is emptied

clean tank: pressure wash if possible.

AquacamD Power Section

grease top unit: 18 pumps

grease lower unit: until grease discharges from outlet opposite grease pip.

Cover securely with tarp.

Decanter Valve

leave open

Decanter Weir

leave open

inspect seal: put on maintenance list for spring if necessary.

remove decant hose from bottom; store hardware

Sludge Pump

remove

Plug

remove

5. Chlorine Contact TanksEdit

Part

Maintenance

Completed

Initials

Sumps

remove plugs

Outside Drainage

remove caps

Disinfection

remove both bleach lines

coil and zip tie for storage

Dechlorination

remove sodium bisulfite line

coil and zip tie for storage

6. Effluent Pump StationEdit

Part

Maintenance

Completed

Initials

Effluent Pump Station

open effluent line valve at northeast corner of EQ

Clean EPS with salt or freshwater

Pump out as much water as possible

remove effluent pump

open EPS valve (outside, at bottom)

7. Sludge TanksEdit

Part

Maintenance

Completed

Initials

sludge pumps

remove

remove green hoses, clean, and store (alley)

sludge tanks

cap

place cinderblocks on top to keep in place

8. Sludge LinesEdit

1. Leave valves open for winter.

9. Drying BedsEdit

Part

Maintenance

Completed

Initials

Drying Beds

Screw tops to baes

sludge lines

open all valves

well

Remove pump; store

remove green hose; store

supernatant lines

close ball valve

remove check valve

drain line

leave ball valve open

10. Effluent LineEdit

Part

Maintenance

Completed

Initials

outfall

remove

coordinate w/island engineer

11. Drying Bed Supernatant WellEdit

1. Remove and store pump.

12. BiosolidsEdit

Part

Maintenance

Completed

Initials

have waste management empty dumpster

put hold on account

Morning RoundsEdit

Standard Operating Procedures

Summary of Method

¨ Monitors pump station hours and alarms, EQ and SBR levels, and chemical levels.

Health and Safety

¨ Exposure to contaminants is limited by the use of the following personal protective equipment: latex gloves.

¨ Wash hands thoroughly after possible exposure to contaminants.

¨ To limit exposure to chemicals wear nitrile gloves.

¨ MSDS sheets for bleach and sodium bisulfite are kept in the SICWTF lab; these can be used to answer specific questions.

Apparatus and Materials

¨ Yard stick.

¨ Hook to trigger pump station alarms.

Frequency of Analysis

¨ Daily

Sample Handling and Documentation Procedures

¨ Record time of analysis and analyst(s) initials on morning rounds benchsheet.

¨ Fill in all fields. Do not leave any blank.

Analysis Procedure

Main pump station:

1. Hours

a. Pump timers are located in the breaker box in the engineering office.

2. Alarm:

a. The main pump station is located behind the truck trestle. .

b. Open the top.

c. Using the hook, completely overturn the topmost float switch.

Doctor’s pump station

1. Hours

a. Pump timers are located in breaker box under the northwest corner of Doctor’s.

2. Alarm

a. The doctor’s pump station is located near the road to the northwest of the doctor’s building.

b. Open the top.

c. Using the hook, completely overturn the topmost float switch.

Effluent Pump Station

1. Hours

a. Pump timer is located on the main control table in the SICWTF garage.

2. Alarm

a. Wearing gloves, reach in and turn over top float switch.

EQ and SBR

1. Read levels from measuring sticks secured in tanks.

Chemical Levels

1. Always wear nitrile gloves when handling chemicals.

a. Wear respirator if irritated by chemicals.

2. Chlorine

a. Open second opening on barrel.

b. Insert measuring stick.

c. Read level.

i. Wipe off measuring stick.

d. Securely close opening.

3. Sodium Bisulfite

a. Open second opening on barrel.

b. Insert measuring stick.

c. Read level.

i. Wipe off measuring stick.

d. Securely close opening.

Edit

Process TestingEdit

Process Test SamplingEdit

Standard Operating Procedures

Summary of Method

¨ Describes method used to sample mixed liquor for the following analyses: MLSS, pH, OUR, microscopic examination, nitrates.



Sample Preservation, Containers, and Storage

¨ Samples are collected using a sludge judge.

¨ Samples are temporarily stored in a plastic bucket before redistribution to other sampling containers for individual analysis.



Health and Safety

¨ When handling mixed liquor, use the following personal protective gear to minimize exposure to contaminants: latex gloves.

¨ Wash hands thoroughly after possible exposure to contaminants.




Apparatus and Materials

¨ Sludge Judge

¨ Plastic bucket




Frequency of Sampling

¨ Daily




Sample Type

¨ Grab




Sample Location

¨ SBR




Sample Collection Procedures

1. Sample from the SBR during the last few minutes of React.



2. Use sludge judge to collect sample.



3. Dip a few feet of the sludge judge into the mixed liquor.

a) The foot valve on the sludge judge will effectively draw a sample.

b) Lift the sludge judge out of the mixed liquor, submerse the sludge judge in the mixed liquor, lift the sludge judge, submerse, etc.

i) Repeat until adequate sample is drawn.



4. Place the end of the sludge judge into the bucket.

a) Slowly drain the tube by pressing the valve into the bottom of the bucket.




Sample Handling and Documentation

1. Time of sample is recorded on appropriate bench sheet.

a) Include height of SBR at time of sample.



2. For process tests, sub-sample from this mixed liquor sample.












Process Tests

Dissolved Oxygen (DO) ProfileEdit

Standard Operating Procedures

Summary of Method

¨ Measures dissolved oxygen levels in SBR Basin

¨ With other process results, provides an indication of what is happening in the SBR basin.

¨ Can be used to adjust aeration times and cycle lengths.




Sample Preservation, Containers, and Storage

¨ N/A




Health and Safety

¨ Exposure to contaminants is limited by the use of the following personal protective equipment: latex gloves.



¨ Wash hands thoroughly after possible exposure to contaminants.




Interferences

¨ N/A




Apparatus and Materials

¨ YSI 55 DO meter and probe.




Reagents

¨ N/A




Frequency of Analysis

¨ Daily



Sample Type

¨ N/A




Sampling Location

¨ SBR Basin




Sample Collection Procedures

¨ N/A




Sample Handling and Documentation Procedures

¨ N/A




Analysis Procedure

1. Place dissolved oxygen probe in the SBR.

a) Leave meter on catwalk next to tank.

i) Wrap probe cord around railing post to keep meter from falling in.

b) Make sure probe is submerged in mixed liquor, beneath any foam.



2. A complete dissolved oxygen profile is the most useful

a) Beginning in mix fill and continuing through the end of decant.

b) Measurements approximately every 5 minutes.



3. When complete, remove probe from SBR and store in lab.

a) Wipe with paper towel.

b) Remove hair and large particles from probe.

c) Wipe everything down with bleach before placing in lab.




Quality control

¨ DO meter calibrated before each use.

¨ Preventative maintenance schedule outlined in the QAQC followed.




Corrective Actions



¨ Probe membrane changed if necessary.

¨ Corrective actions outlined in “Corrective Action Contingencies” section of this QA/QC manual are followed

Safety, Pollution Prevention, and Waste Management

¨ N/A




References



YSI 55 Dissolved Oxygen meter and probe instruction booklet

Total Iron mg/LEdit

Standard Methods 18th edition: 3500–Fe D

Standard Operating Procedures

Summary of Method

¨ Iron is a limiting nutrient in the metabolism of BOD.

¨ Iron in the EQ is measured and used with influent BOD values to determine a goal concentration of iron.

¨ Ferrous Sulfate is added to achieve desired iron concentrations.




Sample preservation, Containers, and Storage

¨ Samples are collected in polyethylene beaker.

¨ Samples are analyzed immediately so they do not have to be stored in an acidic solution.

¨ Samples are not stored.




Health and Safety

¨ Exposure to contaminants is limited by the use of the following personal protective equipment: latex gloves.



¨ Wash hands thoroughly after sample collection and analysis.

Interferences

¨ See referenced methods for possible interferences.

¨ MSDS sheets for all chemicals are kept on file in the SICWTF lab; these can be used as a reference to answer specific questions.




Apparatus and Materials

¨ Hach DR/850 Colorimeter set on Program 33: iron analysis

¨ 50 or 100 mL polypropylene beakers.

¨ 10 mL sample cells and caps.

¨ 2mL pipette and pipettor

¨ Accuwipes (or comparable “delicate task wipes”)




Reagents

¨ FerroVer Iron Reagent

¨ Deionized (DI) laboratory water




Frequency of Analysis

¨ Daily




Sample Type

¨ Grab




Sampling Location

¨ Equalization (EQ) basin




Sample Collection Procedures

1. Use sludge nabber to grab influent sample from the EQ.

a) Rinse sludge nabber in influent 3 times before grabbing sample.

b) Rinse beaker with influent 3 times before collecting sample.




Sample Handling and Documentation Procedures

¨ Sample time and sampler(s)’ initials are recorded on appropriate bench sheet.




Analysis Procedure

1. Triple rinse two sample cells and caps with DI water: sample and blank



2. Pour 10 mL of sample into each sample cell.

a) Be sure to measure from the bottom of the meniscus.

b) Place one in front of the other on the bench top with the sample behind.

i) The sample behind will be the test cell and the sample in front will be the blank.

3. Add a reagent packet to the test cell.

a) Cover both sample cells.

b) Gently shake test cell to mix reagent.



Analysis Procedure Continued



4. Turn on colorimeter.

a) Ensure that the correct program is active.

b) Press timer.

c) When 3:00 appears on the screen, press enter to begin.



5. Wipe the sample cells with an Accuwipe to remove drips or fingerprints.



6. When colorimeter alarm sounds after 3 minutes, place the blank in colorimeter.

a) Place colorimeter cap over sample cell.

b) Press the “zero” button.

c) When display reads 0.00, remove the blank.



7. Place test cell in the colorimeter.

a) Cover and press “read”



8. Record results and analyst(s)’ initials on the appropriate bench sheet.



9. Calculate grams of Ferrous Sulfate to be added to the EQ.

a) Equations follow.




Equations

1. Desired concentration (mg/L) = Influent BOD (mg/L)/ 200



2. Change desired (mg/L) =

desired concentration (mg/L) – measured concentration (mg/L)



3. EQ volume (Mgal) =

(EQ level (ft) x 16 (ft) x 24 (ft) x 7.48 (gal/ft3))/1,000,000



4. Fe required (lbs) =

Change desired (mg/L) x 8.34(lb * L/Mgal * mg) x EQ volume (Mgal)



5. FeSO4 (g) = Fe required (lbs) x (1lb FeSO4/0.37lbFe) x (454g/lb)




* Simplified: FeSO4 (g) = change (mg/L) x EQ level (ft) x 29.394




Quality Control

¨ N/A



Corrective Actions

¨ N/A




Safety, Pollution Prevention, and Waste Management

¨ All sample waste can be poured down the drain.

¨ Return remaining sample to the EQ.




References

Standard Methods 18th edition: 3500–Fe D

Code of Federal Regulations: Table II – Required Containers, Preservation Techniques, and Holding Times

Hach DR/850 Colorimeter Handbook




Mixed Liquor Suspended SolidsEdit

Standard Operating Procedures

Summary of Method

¨ This process test estimates the strength of the bacterial population in the SBR.

¨ With SBR volume, is used to calculate bacterial mass.

¨ With influent BOD volumes and feeding volumes, allows calculation of food to mass ratio.

Sample Preservation, Containers, and Storage

¨ MLSS analysis must begin within 7 days of the last composite sample time.

¨ Composite samples are collected unpreserved in polyethylene or glass beakers.

¨ Composites are stored in polyethylene or glass bottles.

¨ Composites are preserved cool at <6˚C.

Health and Safety

¨ To limit exposure to chemicals, use the following personal protective equipment: latex gloves.

¨ MSDS sheets for all chemicals are kept on file in the SICWTF lab; these can be used as a reference to answer specific questions.

Interferences

¨ See referenced method(s) for interferences.

Apparatus and Materials

¨ TSS Oven Model 10

¨ ANDHR60 Balance

¨ Gast 1/3 hp Vacuum Pump

¨ Forceps

¨ Porcelain Buchner funnel for 12.5cm paper

¨ 2 500mL glass suction flasks

¨ 100mL graduated cylinder

¨ 12.5cm glass microfiber filter

¨ De-ionized (DI) laboratory water

Reagents

¨ N/A

Frequency of Analysis

¨ At least 3 tests each week.

¨ Preferably daily.

Sample Type

¨ Grab

Sampling Location

¨ SBR

Sample Collection Procedures

1. See process sampling procedures (pg. 38)

2. Mixed liquor transferred from sample bucket to polyethylene bottle.

Sample Handling and Documentation Procedures

1. Sample bottles are labeled with the following.

a) Type of sample.

b) Test to be performed.

c) Initials of sampler.

b) Sample time and date.



2. Samples are immediately refrigerated.

Filter Apparatus Set-up

1. Plug vacuum pump in.



2. Connect suction flask with attached vacuum tubing to intake (right side) of vacuum pump.



3. Connect 2nd suction flask to first with attached vacuum tubing and rubber stopper.

b) Fit Buchner funnel to 2nd suction flask.

Filter Pre-Washing

1. Set up filter apparatus.



2. Triple rinse graduated cylinder and Buchner funnel with DI water.



3. Remove filter and set in place on Buchner funnel.

a) Wet the filter surface with DI water and turn the pump on.

i) Fit filter into the Buchner funnel.

Be very careful not to tear.



4. Rinse each filter three times with 20mL of DI water.

a) Use 100mL graduated cylinder triple-rinsed with DI water.

5. Turn pump off when surface appears dry.

a) Remove filter with forceps and place in oven.

i) Note time in logbook.

Filter Weighing



1. When weighing, turn off air conditioning and close lab doors.



2. Filters must remain in oven for at least two hours.

a) Prior to weighing, filters are placed in dessicator.

i) Filters must remain in dessicator for at least one hour.

3. Move filters back to oven in between weighings.



4. Make sure balance is level before weighing.

a) Air bubble in level on back of balance is in center of circle.



5. Shut all balance doors: sides and top.



Filter Weighing Continued



6. Press the re-zero button to zero the balance.

a) Make sure the balance reads 0.0000 before each weighing.

7. Take filter out of dessicator.

a) Place on balance.

b) Close balance doors.

c) Wait until reading stabilizes.

i) Record weight in appropriate spot on benchsheet.



8. Zero balance between weighings.

Analysis Procedure

1. Pre-wash all filters according to filter Pre-washing procedures.



2. Weigh all filters according to Filter Weighing and Check Weigh procedures.

a) Record weights under “Dry Weight”.



3. Check “Dry Weights”.

a) Must be within 0.0005g of original dry weight.

i)If not, cycle filters in question back through the oven and dessicator until two consecutive weights are within 0.0005.

Use first of those two weights as “Dry Weight”.



4. Fill in all sampling information on bench sheet.



5. Triple rinse graduated cylinder and Buchner funnel with DI water.



6. Set up filtering apparatus.



7. Place filter on Buchner funnel

a) Wet with DI water and press carefully into place.

i) If filter is torn, discard and use extra.



8. Shake sample vigorously and immediately measure into 100mL graduated cylinder.

a) 75mL

i) reduce volume if necessary



9. Record actual sample volume on bench sheet.



10. Turn on vacuum pump.



Analysis Procedure Continued



11. Pour sample carefully over filter.



12. Rinse graduated cylinder three times with 30mL DI water.



13. Rinse down sides of funnel.



14. Turn pump off.



15. Carefully remove filter with forceps.

a) Lift and hold filter by edges so no solids are removed.

i) If there are visible cracks or tears in the filter, repeat the sample with one of the extra filters.

b) Place the sample on in correctly labeled spot on filter in oven.



16. When last filter is placed in oven, record time in logbook.



17. After filters have dried (>2hrs), weigh all filters according to Filter Weighing and Check Weigh procedures.

a) Record weights under “Final Weight”



Equations and Calculations

Dry Solids Weight(g) = Final Weight(g)-Dry Weight(g)

(do not use check final weight or check dry weight in calculations)



MLSS(mg/L) = (dry solids (g) x 1000mg/L x 1000mg/L)/mL sample




Safety, Pollution Prevention, and Waste Management

¨ All sample waste can go down the drain.

¨ Filters can be disposed of in the trash.

References

ANDHR60 Balance Manual.

Code of Federal Regulations: Table II – Required Containers, Preservation

Standard Methods, 18th Edition: Solids 2540 D



Waste Activated SludgeEdit

Standard Operating Procedures

Summary of Method

¨ This process test estimates the life cycle of bacteria.

¨ Mean Cell Retention Time (MCRT) can be calculated from MLSS concentration, WAS concentration, and sludge volume wasted each day.

¨ MCRT manipulated to control bacterial populations in the process.

Sample Preservation, Containers, and Storage

¨ WAS analysis must begin within 7 days of the last composite sample time.

¨ Composite samples are collected unpreserved in polyethylene or glass beakers.

¨ Composites are stored in polyethylene or glass bottles.

¨ Composites are preserved cool at <6˚C.

Health and Safety

¨ To limit exposure to chemicals, use the following personal protective equipment: nitrile gloves.

¨ MSDS sheets for all chemicals are kept on file in the SICWTF lab; these can be used as a reference to answer specific questions.

Interferences

¨ See referenced method(s) for interferences.

Apparatus and Materials

¨ TSS Oven Model 10

¨ ANDHR60 Balance

¨ Gast 1/3 hp Vacuum Pump

¨ Forceps

¨ Porcelain Buchner funnel for 12.5cm paper

¨ 2 500mL glass suction flasks

¨ 100mL graduated cylinder

¨ 12.5cm glass microfiber filter

¨ De-ionized (DI) laboratory water

Reagents

¨ N/A

Frequency of Analysis

¨ At once each week.

¨ Preferably three times each week.

Sample Type

¨ Grab

Sampling Location

¨ Sludge tank inflow

Sample Collection Procedures

1. Use sludge nabber to sample from sludge tank inflow during sludge wasting.

a) last few minutes of decant.

2. Transfer sludge to polyethylene bottle for storage.

Sample Handling and Documentation Procedures



1. Sample bottles are labeled with the following.

a) Type of sample.

b) Test to be performed.

c) Initials of sampler.

b) Sample time and date.



2. Samples are immediately refrigerated.

Filter Apparatus Set-up

1. Plug vacuum pump in.



2. Connect suction flask with attached vacuum tubing to intake (right side) of vacuum pump.



3. Connect 2nd suction flask to first with attached vacuum tubing and rubber stopper.

b) Fit Buchner funnel to 2nd suction flask.

Filter Pre-Washing

1. Set up filter apparatus.



2. Triple rinse graduated cylinder and Buchner funnel with DI water.



3. Remove filter and set in place on Buchner funnel.

a) Wet the filter surface with DI water and turn the pump on.

i) Fit filter into the Buchner funnel.

Be very careful not to tear.



4. Rinse each filter three times with 20mL of DI water.

a) Use 100mL graduated cylinder triple-rinsed with DI water.

5. Turn pump off when surface appears dry.

a) Remove filter with forceps and place in oven.

i) Note time in logbook.

Filter Weighing



1. When weighing, turn off air conditioning and close lab doors.



2. Filters must remain in oven for at least two hours.

a) Prior to weighing, filters are placed in dessicator.

i) Filters must remain in dessicator for at least one hour.

3. Move filters back to oven in between weighings.



4. Make sure balance is level before weighing.

a) Air bubble in level on back of balance is in center of circle.



5. Shut all balance doors: sides and top.

Filter Weighing Continued



6. Press the re-zero button to zero the balance.

a) Make sure the balance reads 0.0000 before each weighing.

7. Take filter out of dessicator.

a) Place on balance.

b) Close balance doors.

c) Wait until reading stabilizes.

i) Record weight in appropriate spot on benchsheet.



8. Zero balance between weighings.

Analysis Procedure

1. Pre-wash all filters according to filter Pre-washing procedures.



2. Weigh all filters according to Filter Weighing and Check Weigh procedures.

a) Record weights under “Dry Weight”.



3. Check “Dry Weights”.

a) Must be within 0.0005g of original dry weight.

i)If not, cycle filters in question back through the oven and dessicator until two consecutive weights are within 0.0005.

Use first of those two weights as “Dry Weight”.



4. Fill in all sampling information on bench sheet.



5. Triple rinse graduated cylinder and Buchner funnel with DI water.



6. Set up filtering apparatus.



7. Place filter on Buchner funnel

a) Wet with DI water and press carefully into place.

i) If filter is torn, discard and use extra.



8. Shake sample vigorously and immediately measure into 100mL graduated cylinder.

a) 75mL

i) reduce volume if necessary



9. Record actual sample volume on bench sheet.



10. Turn on vacuum pump.



Analysis Procedure Continued



11. Pour sample carefully over filter.



12. Rinse graduated cylinder three times with 30mL DI water.



13. Rinse down sides of funnel.



14. Turn pump off.



15. Carefully remove filter with forceps.

a) Lift and hold filter by edges so no solids are removed.

i) If there are visible cracks or tears in the filter, repeat the sample with one of the extra filters.

b) Place the sample on in correctly labeled spot on filter in oven.



16. When last filter is placed in oven, record time in logbook.



17. After filters have dried (>2hrs), weigh all filters according to Filter Weighing and Check Weigh procedures.

a) Record weights under “Final Weight”



Equations and Calculations

Dry Solids Weight(g) = Final Weight(g)-Dry Weight(g)

(do not use check final weight or check dry weight in calculations)



WAS total suspended solids (mg/L) =

(dry solids (g) x 1000mg/L x 1000mg/L)/mL sample




Safety, Pollution Prevention, and Waste Management

¨ All sample waste can go down the drain.

¨ Filters can be disposed of in the trash.

References

ANDHR60 Balance Manual.

Code of Federal Regulations: Table II – Required Containers, Preservation

Standard Methods, 18th Edition: Solids 2540 D


Nitrate Edit

Nitrate as N (mg/L) Colorimetric Method

Standard Methods, 18th edition: 352.1

Standard Operating Procedures

Summary of Method

¨ This test determines the amount of nitrates in mixed liquor and effluent.

¨ Allows operators to determine how the nitrogen cycle is affecting the biological process in the SBR.




Sample Preservation, Containers, and Storage

¨ Samples are collected in polyethylene beakers.

¨ If samples are stored, they are kept for 48 hours in a cool environment, <6° C.

¨ At the SICWTF, samples are immediately analyzed for nitrates.




Health and Safety

¨ To limit exposure to chemicals, use the following personal protective equipment: nitrile gloves.

¨ MSDS sheets for all chemicals are kept on file in the SICWTF lab; these can be used as a reference to answer specific questions.




Interferences

¨ See referenced methods for interferences.




Apparatus and Materials

¨ Hach DR/850 Colorimeter set on Program 51: Nitrate analysis

¨ 50 or 100 mL polypropylene beakers.

¨ 10 mL sample cells and caps.

¨ 2mL pipette and pipettor

¨ Accuwipes (or comparable “delicate task wipes”)




Reagents

¨ NitraVer Nitrate Reagent

¨ De-ionized (DI) laboratory water



Frequency of Analysis

¨ Daily




Sample Type

¨ Grab




Sampling Location

¨ Mixed liquor: SBR

¨ Effluent: Effluent Pump Station (EPS)




Sample Collection Procedures

¨ Mixed liquor: see process sample collection (pg. 38)

¨ Effluent: Triple rinse beaker with DI water before sampling.

Triple rinse beaker in effluent before grabbing sample.




Sample Handling and Documentation Procedures

¨ Sample time and sampler(s)’ initials are recorded on appropriate bench sheet.




Analysis Procedure

Mixed liquor:



1. Transfer approximately 100mL mixed liquor from process sample to 100 mL beaker.



2. Set up filtering apparatus for 12.5cm filters

a) See Total Suspended Solids SOP.



3. Filter the mixed liquor.

a) Dispose of the filter.



4. Triple rinse all glassware with DI water.

a) beaker.

b) two sample cells: sample and blank.



5. Transfer filtrate to beaker.

Analysis Procedure Continued



6. Fill a pair of sample cells to the 10 mL mark with filtrate: be sure to read from the bottom of the meniscus.

a) place one in front of the other on the bench top with the sample behind.

i) The sample cell behind will be the test cell and the sample cell in front will be the blank.



7. Add a reagent packet to the test cell.

a) Cover both sample cells.

b) Gently shake test cells to mix reagent.



6. Turn on colorimeter.

a) Ensure that the correct program is active.

b) Press timer. When 1:00 appears on the screen, press enter to begin.



8. Shake test cell until the colorimeter alarm sounds.



9. 5:00 should appear on the screen.

a) replace the test cell on the bench top.

b) press enter to begin timing.



10. Wipe all sample cells with an Accuwipe to remove drips or fingerprints.



11. When colorimeter alarm sounds after 5 minutes, place blank in colorimeter.

a) Place colorimeter cap over sample cell.

b) Press the “zero” button.

c) When display reads 0.00, remove the blank.



12. Place test cell in the colorimeter.

a) Cover and press “read”

b) Record result on bench sheet along with time of analysis and analyst initials.






Effluent:

1. After sampling, follow steps 6 through 12, using effluent instead of filtrate.




Quality Control

¨ N/A



Corrective Actions

¨ N/A




Safety, Pollution Prevention, and Waste Management

¨ All sample waste can be poured down the drain.



References

Code of Federal Regulations: Table II – Required Containers, Preservation Techniques, and Holding Times

Hach DR/850 Colorimeter

Standard Methods, 18th Edition: 352.1



TurbidityEdit

Standard Operating Procedures

Summary of Method

¨ Provides an estimate of Total Suspended Solids in effluent.




Sample Preservation, Containers, and Storage

¨ Turbidity samples are collected in polyethylene beakers.

¨ If samples are stored, they are kept for 48 hours in a cool environment, <6° C.

¨ At the SICWTF, samples are immediately analyzed for turbidity.

¨ Results will not be accurate if samples are allowed to settle.




Health and Safety

¨ Exposure to contaminants is limited by the use of the following personal protective equipment: latex gloves.

¨ Wash hands thoroughly after sample collection and analysis.




Interferences

¨ N/A




Apparatus and Materials

¨ Hach DR/850 Colorimeter set on Program 95: turbidity analysis

¨ 50 or 100 mL polypropylene beakers.

¨ 10 mL sample cells and caps.

¨ Accuwipes (or comparable “delicate task wipes”)




Reagents

¨ De-ionized (DI) laboratory water




Frequency of Analysis

¨ Daily



Sample Type

¨ Grab



Sampling Location

¨ Effluent Pump Station (EPS) inflow.



Sample Collection Procedure

¨ Triple rinse beaker in DI water

¨ Triple rinse beaker in effluent before grabbing sample




Sample Handling and Documentation Procedures

¨ Sample time and sampler(s)’ initials are recorded on appropriate bench sheet.




Analysis Procedure

1. Triple rinse two sample cells and caps with DI water: sample and blank



2. Pour 10 mL DI water into one sample cell and cap.

a) Be sure to measure from the bottom of the meniscus.



3. Pour 10 mL of sample into other sample cell and cap.

a) Be sure to measure from the bottom of the meniscus.

b) Place one in front of the other on the bench top with the sample behind.

i) The sample cell behind is the test cell and the sample in front (DI water) will be the blank.



5. Turn on colorimeter.

a) Ensure that the correct program is active.



6. Wipe the sample cells with an Accuwipe to remove drips or fingerprints.



7. Place the blank in colorimeter.

a) Place colorimeter cap over sample cell.

b) Press the “zero” button.

c) When display reads 0.00, remove the blank.



8. Place test cell in the colorimeter.

a) Cover and press “read”



9. Record results and analyst(s)’ initials on the appropriate bench sheet.

Quality Control

¨ N/A




Corrective Actions

¨ N/A




Safety, Pollution Prevention, and Waste Management

¨ All sample waste can be poured down the drain.




References

Code of Federal Regulations: Table II – Required Containers, Preservation Techniques, and Holding Times

Hach DR/850 Colorimeter



SalinityEdit

Standard Operating Procedures

Write this next year when have access to manual.

Need salinity to calibrate the field DO meter.

Should also include in calibration of bench top DO meter.

Useful to monitor fluctuations in salinity.

Oxygen Uptake Rate (OUR)Edit

Standard Operating Procedures

Summary of Method

¨ Indication of bacterial activity in the SBR basin.

¨ Positive OUR indicates living bacteria.

¨ Higher OUR results indicate that all BOD has not been consumed during react.

¨ With other process results, OUR helps determine aeration timers and cycle lengths.




Sample Preservation, Containers, and Storage

¨ Mixed liquor sample is transferred to 1L plastic bottle.

¨ Samples are not stored.




Health and Safety

¨ Exposure to contaminants is limited by the use of the following personal protective equipment: latex gloves.



¨ Wash hands thoroughly after sample collection and analysis.

Interferences

¨ N/A




Apparatus and Materials

¨ YSI 5100 Benchtop DO Meter

¨ YSI 5905/5010 BOD probe

¨ 1L plastic bottle.

¨ BOD bottle

¨ De-ionized (DI) laboratory water




Reagents

¨ N/A




Frequency of Analysis

¨ Daily




Sample Type

¨ Grab




Sample Location

¨ SBR Basin

Sample Collection Procedures

¨ See process sample collection section (pg. 38)




Sample Handling and Documentation Procedures

1. Sample time and sampler(s)’ initials are recorded on appropriate bench sheet.



2. Mixed liquor samples are transferred from collection bucket to 1L plastic bottle.

a) Plastic bottle is filled approximately ¾ full with mixed liquor.

b) Leave at least 1” air at top of bottle.

c) Tightly cover bottle.



Analysis Procedure

1. Follow all calibration procedures for the DO meter prior to analysis.



2. Set-up the DO meter for OUR analysis.

a) Navigate to the applications menu using the mode key.

b) Select OUR (leftmost soft key) to enter OUR mode.



3. Triple rinse BOD bottle with DI water.



4. Saturate mixed liquor sample with air.

a) Vigorously shake bottle of mixed liquor.

b) Open bottle to let air in.

c) Close tightly.

d) Vigorously shake bottle.



Analysis Procedure Continued



5. Pour sample into prepared BOD bottle.

a) Fill to top (until overflows).



6. Place BOD bottle next to DO meter.

a) Several paper towels under bottle.



7. Place BOD probe in bottle.

a) Turn on stirrer.



8. When value on display become positive, press the start soft key.




9. This analysis is complete after 10 minutes.

a) The meter will keep track of time.

b) OUR value will be displayed on screen.



10. Record OUR value in mg/L/hr on appropriate benchsheet.



11. Thoroughly clean BOD probe with DI water.

a) Remove hairs or large particles from stirrer by hand.

b) If necessary, pull stirrer off to clean.



12. Place BOD probe in storage bottle.



13. Turn DO meter off.




Quality control

¨ DO meter calibrated before each use.

¨ Preventative maintenance schedule (pg. 21) followed.




Corrective Actions



¨ Membrane cap changed if necessary.

¨ Corrective actions outlined in “Corrective Action Contingencies” section of this QA/QC manual are followed



Safety, Pollution Prevention, and Waste Management

¨ Mixed liquor sample should be poured in EQ basin when analysis is complete.




References

YSI 5100 Benchtop DO Meter Manual

YSI 5905/5010 BOD probe Manual

Thirty Minute SettleabilityEdit

Standard Operating Procedures

Summary of Method

¨ This is a rough indication of settling in the SBR.

¨ Allows operators to see changes in the process that effect settling immediately.

¨ Filament growth and short-term toxic effects are easily seen in a settleometer.




Sample Preservation, Containers, and Storage

¨ Mixed liquor sample is transferred to plastic settleometer.

¨ Samples are not stored.




Health and Safety

¨ Exposure to contaminants is limited by the use of the following personal protective equipment: latex gloves.

¨ Wash hands thoroughly after sample collection and analysis.




Interferences

¨ N/A




Apparatus and Materials

¨ Settleometer.

¨ Stirring paddle.

Reagents

¨ N/A




Frequency of Analysis

¨ Daily




Sample Type

¨ Grab




Sampling Location

¨ SBR basin.




Sample Collection Procedures

¨ See process sample collection section (pg. 38)




Sample Handling and Documentation Procedures

1. Sample time and sampler(s)’ initials are recorded on appropriate bench sheet.



2. Mixed liquor samples are transferred from collection bucket to settleometer.

a) Settleometer is filled with 1L of mixed liquor.



Analysis Procedure

After mixed liquor has been transferred to the settleometer:



1. Place settleometer on bench top in lab.

a) Place several paper towels under.



2. Place the stirring paddle in the settleometer.

a) Stir contents thoroughly.

b) Remove stirring paddle.

c) Note time the sample was stirred.



3. Thirty minutes after stirring, observe the following

a) Settled solids

b) Floating solids

i) Measure volumes based on graduations on side of settleometer.



4. Record volumes in appropriate bench sheet.




Quality Control

¨ N/A

Corrective Actions

¨ N/A




Safety, Pollution Prevention, and Waste Management

¨ Mixed liquor sample should be poured in EQ basin when analysis is complete.





Mixed Liquor pHEdit

Standard Methods, 18th edition: pH value (4500-H+)/Electrometric Method

Standard Operating Procedures

Summary of Method

¨ This test determines pH levels of mixed liquor.

¨ pH should be 6.5 – 8.0 su

Required Containers, Preservation Techniques, and Holding Times



¨ pH analysis must be complete within 15 minutes of sampling.

¨ Mixed liquor samples are transferred to polyethylene beaker.

¨ Samples are not stored.



Health and Safety



¨ Exposure to contaminants is limited by the use of the following personal protective equipment: latex gloves.

¨ Wash hands thoroughly after sample collection and analysis.



Interferences

¨ Temperature.

¨ Sodium error at pH>10.

¨ See referenced method(s) for additional interferences.



Apparatus and Materials

¨ pH Meter Thermo Orion 420+

¨ pH Electrode Orion Triode 9157BN

¨ 50 or 100 mL polypropylene beaker

¨ magnetic stirrer

¨ De-ionized (DI) laboratory water.

¨ Accuwipes (or comparable “delicate task wipes”)

Reagents

¨ N/A

Frequency of Analysis

¨ Daily

Sample Type

¨ Grab sample.

Sampling Location

¨ SBR



Sample Collection Procedures

¨ See “Process Test Sampling Procedures” (pg. 38)

Sample Handling and Documentation Procedures

¨ Sample time and sampler initials are recorded on appropriate bench sheet.

Analysis Procedure

After mixed liquor sample has been transferred to clean, triple-rinsed beaker:



1. Place clean, triple rinsed, dry stirring rod in sample.



2. Turn on pH meter.

a) Should have been standardized within 24 hours.

b) Resolution should be set to one decimal place.



3. Rinse electrode thoroughly with DI water.

a) Remove any drips with an accu-wipe.

4. Immerse probe tip in sample.

a) Do not let stirring rod touch probe tip.



5. When display shows blinking ready in upper right corner:

a) Record pH and temperature on appropriate benchsheet.






Quality Control



1. pH meter calibrated to the hundredth of a standard unit (su) on a daily basis.

a) Standards prepared each week.

i)Double batch prepared, but small amount poured into beaker for calibration purposes.

b) Calibration checked with additional standard.

i) Must be within range listed by manufacturer.



Corrective Actions



1. Corrective actions in referenced EPA method are followed.

2. Corrective actions outlined in “Corrective Action Contingencies” section of this QA/QC manual are followed.

Safety, Pollution Prevention and Waste Management

¨ Mixed liquor should be poured into EQ when analysis is complete.




References

Standard Methods, 18th Edition: pH value (4500-H+)/Electrometric Method

pH Meter Thermo Orion 420+ instruction manual

pH Electrode Orion Triode 9157BN instruction manual

Process DataEdit

1. Process data is entered into excel spreadsheets.

a) Emailed to Bob Urban on a weekly basis.

b) DO profiles are graphed.



2. Project for 2009: create excel spreadsheets with process calculations.